Molecular Biology

Replication occurs in the cytoplasm

  1. There is attachment to host receptors, which mediates the endocytosis of the virus into host cell.
  2. There is uncoating and subsequent release of the viral genomic RNA into the cytoplasm
  3. The RNA is translated into two polyproteins to create replication proteins, which happens in the Endoplasmic Reticulum (double stranded RNA genome is created from the positive single stranded RNA).
  4. Subgenomic RNA translation creates the capsid precursor
  5. Assembly of the new virions
  6. Maturation of the capsid and non-lytic virus release

The organization of the astrovirus genome places the Open Reading Frames encoding the structural proteins at the 3’ end and the nonstructural proteins at the 5’ end. Other distinctive features of the family include the lack of a DNA-helicase, and the usage of ribosomal frameshifting as a mechanism to translate the RNA –dependent RNA polymerase.

Works Cited

“Human Astroviruses.” National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 19 Mar. 2015.

Knipe, David M., and Bernard N. Fields. “Chapter 21: Astroviridae.” Fields’ Virology. 6th ed. Philadelphia: Lippincott Williams & Wilkins, 2013. 609-28. Print.

“ViralZone: Astroviridae.” ViralZone: Astroviridae. ExPASy, n.d. Web. 19 Mar. 2015.

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